Lab log

Lab log 2 should include all available data from time points 0, 1, and 2 (0, 4, and 8 weeks, respectively). Attached are 2 files, one that has the step by step guidelines to the Lab Log and the other contains all the data needed. Let me know if you are unable to find them. Please do not copy anything from any website since the professor checks for plagiarism.

Note: As promised, all of your data in its current form is now posted here on Canvas under Modules in the final presentation module. A few notes:

  • The qPCR data are not complete, but there are quite a few representative sets that are present. For example, all monocultures except for Durisdinium at one time point are present. Remember that this experiment remains under progress and the data are thus still being collected and compiled. Don’t let this frighten you.
  • All cell count data are complete except for 7 points in the final time point. These 7 cultures were restarted one month behind the others, as the originals were compromised in various ways. This is common in science and won’t affect your results or analyses in any large and meaningful ways.
  • The data sheet contains 6 tabs: 3 for symbiont density (cell count results) and 3 for qPCR, all separated by time point and organized by temperature, nitrogen concentration, and genus. pay special attention to the parts which are in green. If you don’t know what a tab is in Excel, please look it up on Google and be sure that you can access all of them. This is critical, as if you only look at the first tab, you will not have anything meaningful to present or write about.
  • Hopefully you have, during your meetings, been working with the non-data portions of your talk, and that this can simply be dropped right into your presentation. I noted during our lab meetings that most of y’all were building a presentation before even leaving, so I am confident in this.
  • Don’t forget to use the rubric as a checklist to get that sweet sweet A!
  • AVERAGES ARE YOUR FRIEND! As always, it is inadvisable to use individual data points in your graphs and graphics. Use averages to point out trends, and don’t forget that you are bound to produce many more graphs than you intend to use, just to investigate and examine the data. (and for goodness sake, please avoid including tables in your presentation!)
  • As far the qPCR data go, remember that these are EXACTLY equivalent to symbiont density data. Just think of the “log[cell count] numbers as your symbiont density, genus-by-genus.

“Some of the formatting moved a few dividers around and made it look odd.

To clarify:

gamma (looks like a little y)=Symbiodinium+Breviolum
psi (looks like a pitchfork)=Durisdinium+Symnbiodinium
phi (cirlce with line through it)=Durisdinium+Breviolum

The polycultures have the following headers in this order:

Genus-Culture Number-Ct-Melt Temperature-log[cell count]-composition-Temperature-Nitrogen

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