Calculated molecular weights for the three DHFR fusion proteins analyzed

SDS-PAGE Gel code blue gel image

Western Blot images for the four primary antibody blots performed

Tag locations for each tag, each of the three DHFR fusion proteins analyzed

Procedures to include that were done for you: SDS-PAGE and Western Blot

Other than preparing your DHFR protein sample for protein loading, provide the basics only – e.g. procedure performed, samples analyzed, and the detection method used, citing the lab manual as described previously

You only need to write procedural stepwise details out for procedures performed yourself (calculation/computational analysis here)

*For this Part II draft, this includes:

Calculations for preparation of GST-DHFR-His gel loading

Calculation of protein molecular weights

Determination of protein tag locations

Include Title page, a draft of the Materials & Methods and Results sections covering SDS-PAGE, Western Blot, and Computational protein analyses, and a Reference section. You do not need to include material from the M&M/Results Part I draft. You will use feedback from part I and II drafts to combine these into your final M&M and Results in sections for the full report.

The Reference section included here should have the Lab Manual referenced in your materials and methods section and any other sources used to write sections of this draft. Written Rough Drafts will be graded on the following:•Complete and accurate content at the time of draft (10pt)•Writing and mechanics: clarity, logical flow of information, spelling/grammar (10pt)•Support and sufficient references (5pt)

The reference for the lab manual: Lab Manual example (be sure to update for your manual version’s specific semester and year):CMMBDepartment. Production of Recombinant Dihydrofolate Reductase. Cell Biology Lab Manual PCB 3023L, Spring2021. University of South Florida, Tampa.