Thin Layer Chromatography (TLC)

1. Which of the compounds listed in the video would best be described as non-polar? (1 pt)

2. Which of the compounds listed in the video would best be described as polar? (1 pt)

3. Explain how TLC separates compounds? (2 points)

4. List the compounds that were found in spinach juice and their R_f values? (2 points)

5. Which compound has a higher R_f value beta-carotene or chlorophyll A? Explain your answer by discussing the molecules functional groups and polarity. (1 point)

5. How would the R_f values for the spinach extract change if the solvent in the developing chamber was more polar? (1 point)

Thin Layer Chromatography (TLC)

Question 1. Why are the spots the same for both the right and left lanes of the TLC plate at Time = 0?  (1 point)

Question 2. Which spot is 3-methyl-1-butanol and which one is 2-phenylacetic acid? Explain how you know this based on polarity. (2 points)

Question 3. What is the new spot that appears during the reaction? (1 point)

Question 4. What has happened to the size of the starting material spots after ~20 minutes? Why? (1 point)

Question 5. How much time did it take to complete the reaction? Explain how you know this. (1 point)

Question 6. Calculate the R_f values for 2-phenylacetic acid, 3-methyl-1-butanol and the both starting materials and the product? Which starting chemical spot is and which one is? (2 points)

Question 7. What can you say about the relative polarities of the starting materials and the product using the Rf values? Explain this by discussing the functional groups on each structure. (2 points)

DETAILED ASSIGNMENT

20201015041729tlc_virtual_lab